Direct probing of the surface ultrastructure and molecular interactions of dormant and germinating spores of Phanerochaete chrysosporium.
Identifieur interne : 000B35 ( Main/Exploration ); précédent : 000B34; suivant : 000B36Direct probing of the surface ultrastructure and molecular interactions of dormant and germinating spores of Phanerochaete chrysosporium.
Auteurs : Y F Dufrêne [Belgique] ; C J Boonaert ; P A Gerin ; M. Asther ; P G RouxhetSource :
- Journal of bacteriology [ 0021-9193 ] ; 1999.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Phanerochaete.
- ultrastructure : Microscopie à force atomique, Phanerochaete, Spores fongiques.
English descriptors
- KwdEn :
- MESH :
- growth & development : Phanerochaete.
- ultrastructure : Phanerochaete, Spores, Fungal.
- Microscopy, Atomic Force.
Abstract
Atomic force microscopy (AFM) has been used to probe, under physiological conditions, the surface ultrastructure and molecular interactions of spores of the filamentous fungus Phanerochaete chrysosporium. High-resolution images revealed that the surface of dormant spores was uniformly covered with rodlets having a periodicity of 10 +/- 1 nm, which is in agreement with earlier freeze-etching measurements. In contrast, germinating spores had a very smooth surface partially covered with rough granular structures. Force-distance curve measurements demonstrated that the changes in spore surface ultrastructure during germination are correlated with profound modifications of molecular interactions: while dormant spores showed no adhesion with the AFM probe, germinating spores exhibited strong adhesion forces, of 9 +/- 2 nN magnitude. These forces are attributed to polysaccharide binding and suggested to be responsible for spore aggregation. This study represents the first direct characterization of the surface ultrastructure and molecular interactions of living fungal spores at the nanometer scale and offers new prospects for mapping microbial cell surface properties under native conditions.
DOI: 10.1128/JB.181.17.5350-5354.1999
PubMed: 10464206
PubMed Central: PMC94041
Affiliations:
- Belgique
- Province du Brabant wallon, Région wallonne
- Louvain-la-Neuve
- Université catholique de Louvain
Links toward previous steps (curation, corpus...)
Le document en format XML
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High-resolution images revealed that the surface of dormant spores was uniformly covered with rodlets having a periodicity of 10 +/- 1 nm, which is in agreement with earlier freeze-etching measurements. In contrast, germinating spores had a very smooth surface partially covered with rough granular structures. Force-distance curve measurements demonstrated that the changes in spore surface ultrastructure during germination are correlated with profound modifications of molecular interactions: while dormant spores showed no adhesion with the AFM probe, germinating spores exhibited strong adhesion forces, of 9 +/- 2 nN magnitude. These forces are attributed to polysaccharide binding and suggested to be responsible for spore aggregation. This study represents the first direct characterization of the surface ultrastructure and molecular interactions of living fungal spores at the nanometer scale and offers new prospects for mapping microbial cell surface properties under native conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10464206</PMID><DateCompleted><Year>1999</Year><Month>10</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9193</ISSN><JournalIssue CitedMedium="Print"><Volume>181</Volume><Issue>17</Issue><PubDate><Year>1999</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of bacteriology</Title><ISOAbbreviation>J Bacteriol</ISOAbbreviation></Journal><ArticleTitle>Direct probing of the surface ultrastructure and molecular interactions of dormant and germinating spores of Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>5350-4</MedlinePgn></Pagination><Abstract><AbstractText>Atomic force microscopy (AFM) has been used to probe, under physiological conditions, the surface ultrastructure and molecular interactions of spores of the filamentous fungus Phanerochaete chrysosporium. High-resolution images revealed that the surface of dormant spores was uniformly covered with rodlets having a periodicity of 10 +/- 1 nm, which is in agreement with earlier freeze-etching measurements. In contrast, germinating spores had a very smooth surface partially covered with rough granular structures. Force-distance curve measurements demonstrated that the changes in spore surface ultrastructure during germination are correlated with profound modifications of molecular interactions: while dormant spores showed no adhesion with the AFM probe, germinating spores exhibited strong adhesion forces, of 9 +/- 2 nN magnitude. These forces are attributed to polysaccharide binding and suggested to be responsible for spore aggregation. 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IdType="pubmed">9665127</ArticleId></ArticleIdList></Reference><Reference><Citation>Biophys J. 1995 May;68(5):1678-80</Citation><ArticleIdList><ArticleId IdType="pubmed">7612810</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1992 Sep 25;257(5078):1900-5</Citation><ArticleIdList><ArticleId IdType="pubmed">1411505</ArticleId></ArticleIdList></Reference><Reference><Citation>Phys Rev Lett. 1986 Mar 3;56(9):930-933</Citation><ArticleIdList><ArticleId IdType="pubmed">10033323</ArticleId></ArticleIdList></Reference><Reference><Citation>Biophys J. 1980 Sep;31(3):425-31</Citation><ArticleIdList><ArticleId IdType="pubmed">7260296</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1994 Nov 4;266(5186):771-3</Citation><ArticleIdList><ArticleId IdType="pubmed">7973628</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1992 Sep 25;257(5078):1944-6</Citation><ArticleIdList><ArticleId IdType="pubmed">1411511</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1978 Apr 13;272(5654):608-10</Citation><ArticleIdList><ArticleId IdType="pubmed">148008</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):836-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11607452</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1988 Jan 15;239(4837):288-90</Citation><ArticleIdList><ArticleId IdType="pubmed">17769992</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1975 Oct 2;257(5525):400-2</Citation><ArticleIdList><ArticleId IdType="pubmed">241021</ArticleId></ArticleIdList></Reference><Reference><Citation>J Struct Biol. 1990 Oct-Dec;105(1-3):54-61</Citation><ArticleIdList><ArticleId IdType="pubmed">2100150</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Rev. 1991 Dec;55(4):684-705</Citation><ArticleIdList><ArticleId IdType="pubmed">1723487</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Microb Physiol. 1993;34:147-202</Citation><ArticleIdList><ArticleId IdType="pubmed">8452092</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1993 Apr;175(7):1946-55</Citation><ArticleIdList><ArticleId IdType="pubmed">8458836</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1991 Sep 20;253(5026):1405-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1910206</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1999 Jan 7;397(6714):50-3</Citation><ArticleIdList><ArticleId IdType="pubmed">9892352</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1985 Aug 13;24(17):4608-18</Citation><ArticleIdList><ArticleId IdType="pubmed">4063343</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1993 Aug;175(16):5135-44</Citation><ArticleIdList><ArticleId IdType="pubmed">8349553</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1995 Apr 7;268(5207):92-4</Citation><ArticleIdList><ArticleId IdType="pubmed">7701347</ArticleId></ArticleIdList></Reference><Reference><Citation>Can J Microbiol. 1994 May;40(5):412-6</Citation><ArticleIdList><ArticleId IdType="pubmed">8069785</ArticleId></ArticleIdList></Reference><Reference><Citation>Ultramicroscopy. 1992 Jul;42-44 ( Pt B):1236-42</Citation><ArticleIdList><ArticleId IdType="pubmed">1413261</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Microb Physiol. 1997;38:1-45</Citation><ArticleIdList><ArticleId IdType="pubmed">8922117</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1976 Feb 19;259(5544):601-3</Citation><ArticleIdList><ArticleId IdType="pubmed">1250410</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1972 Jul 19;273(2):346-58</Citation><ArticleIdList><ArticleId IdType="pubmed">5080323</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province du Brabant wallon</li><li>Région wallonne</li></region><settlement><li>Louvain-la-Neuve</li></settlement><orgName><li>Université catholique de Louvain</li></orgName></list><tree><noCountry><name sortKey="Asther, M" sort="Asther, M" uniqKey="Asther M" first="M" last="Asther">M. Asther</name><name sortKey="Boonaert, C J" sort="Boonaert, C J" uniqKey="Boonaert C" first="C J" last="Boonaert">C J Boonaert</name><name sortKey="Gerin, P A" sort="Gerin, P A" uniqKey="Gerin P" first="P A" last="Gerin">P A Gerin</name><name sortKey="Rouxhet, P G" sort="Rouxhet, P G" uniqKey="Rouxhet P" first="P G" last="Rouxhet">P G Rouxhet</name></noCountry><country name="Belgique"><region name="Région wallonne"><name sortKey="Dufrene, Y F" sort="Dufrene, Y F" uniqKey="Dufrene Y" first="Y F" last="Dufrêne">Y F Dufrêne</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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